Minimal information for studies of extracellular vesicles 2018 (MISEV2018): a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines

Abstract

ABSTRACT The last decade has seen a sharp increase in the number of scientific publications describing physiological and pathological functions of extracellular vesicles (EVs), a collective term covering various subtypes of cell‐released, membranous structures, called exosomes, microvesicles, microparticles, ectosomes, oncosomes, apoptotic bodies, and many other names. However, specific issues arise when working with these entities, whose size and amount often make them difficult to obtain as relatively pure preparations, and to characterize properly. The International Society for Extracellular Vesicles (ISEV) proposed Minimal Information for Studies of Extracellular Vesicles (“MISEV”) guidelines for the field in 2014. We now update these “MISEV2014” guidelines based on evolution of the collective knowledge in the last four years. An important point to consider is that ascribing a specific function to EVs in general, or to subtypes of EVs, requires reporting of specific information beyond mere description of function in a crude, potentially contaminated, and heterogeneous preparation. For example, claims that exosomes are endowed with exquisite and specific activities remain difficult to support experimentally, given our still limited knowledge of their specific molecular machineries of biogenesis and release, as compared with other biophysically similar EVs. The MISEV2018 guidelines include tables and outlines of suggested protocols and steps to follow to document specific EV‐associated functional activities. Finally, a checklist is provided with summaries of key points.

Keywords

Affiliated Institutions

• University of Gothenburg SE
• Harvard University US
• Research Institute for Farm Animal Biology (FBN) DE
• UCLouvain BE
• Centre hospitalier de l'Université Laval CA
• Protéomique, Réponse Inflammatoire et Spectrométrie de Masse FR
• National Tsing Hua University TW
• National Institute on Deafness and Other Communication Disorders US
• Université d'Angers FR
• University of California, Davis US
• La Trobe University AU
• National Institute of Chemistry SI
• University of Science and Culture IR
• University of Amsterdam NL
• PsiOxus Therapeutics (United Kingdom) GB
• Utrecht University NL
• Délégation Paris 5 FR
• MultiMedica IT
• Nationwide Children's Hospital US
• Université Bretagne Loire FR
• University Hospital Bonn DE
• Universidad de Buenos Aires AR
• University of Brescia IT
• Semmelweis University HU
• Cardiff University GB
• Consorzio Interuniversitario per lo Sviluppo dei Sistemi a Grande Interfase IT
• Université Paris Sciences et Lettres FR
• Université de Lille FR
• Hospital Israelita Albert Einstein BR
• Jagiellonian University PL
• Leiden University Medical Center NL
• Ottawa Hospital CA
• Universidade Estadual de Campinas (UNICAMP) BR
• National Research Council IT
• McGill University Health Centre CA
• Australian Institute of Tropical Health and Medicine AU
• Aston University GB
• University of Oxford GB
• École Pratique des Hautes Études FR
• Universitat Politècnica de València ES
• Universidade do Porto PT
• Centro de Investigación Biomédica en Red ES
• University of Malta MT
• Franche-Comté Électronique Mécanique Thermique et Optique - Sciences et Technologies FR
• Paris Cardiovascular Research Center FR
• CIC bioGUNE ES
• ETH Zurich CH
• Universitat Autònoma de Barcelona ES
• Institució Catalana de Recerca i Estudis Avançats ES
• Université de Bordeaux FR
• University of Michigan US
• University of L'Aquila IT
• Oniris FR
• University of Ottawa CA
• Vanderbilt University Medical Center US
• National Interuniversity Consortium of Materials Science and Technology IT
• National Taiwan University Hospital TW
• University of Notre Dame US
• University of Technology Sydney AU
• Université Paris Cité FR
• Johns Hopkins Medicine US
• Hasselt University BE
• Brain Tumour Research GB
• University of Rochester US
• University of Toronto CA
• Medisch Centrum Haaglanden NL
• Universidade de São Paulo BR
• University of Cologne DE
• University of Turin IT
• Oxford Brookes University GB
• Icahn School of Medicine at Mount Sinai US
• Cedars-Sinai Medical Center US
• Université Claude Bernard Lyon 1 FR
• Technical University of Munich DE
• Sorbonne Paris Cité FR
• Karolinska Institutet SE
• University of Edinburgh GB
• Massachusetts General Hospital US
• Centre National de la Recherche Scientifique FR
• Barcelona Institute for Global Health ES
• Ikerbasque ES
• Nantes Université FR
• École Nationale Supérieure de Mécanique et des Microtechniques FR
• Institut d'Investigació en Ciències de la Salut Germans Trias i Pujol ES
• Institut Curie FR
• University of Modena and Reggio Emilia IT
• University of Virginia Health System US
• University of Oulu FI
• University of Colorado Denver US
• James Cook University AU
• Toronto General Hospital CA
• Cornell University US
• Universitat de Barcelona ES
• Memorial Sloan Kettering Cancer Center US
• Cancer Research Institute Ghent BE
• Brigham and Women's Hospital US
• Fundación Ciencias Exactas y Naturales AR
• University of South Alabama US
• Royan Institute IR
• Istituto di Biomedicina e di Immunologia Molecolare Alberto Monroy IT
• Atlantic Cancer Research Institute CA
• University Health Network CA
• Ospedale di Santo Spirito IT
• Mayo Clinic in Florida US
• Lund University SE
• Ghent University Hospital BE
• German Center for Neurodegenerative Diseases DE
• University of Nottingham GB
• National Institutes of Health US
• University of Nebraska Medical Center US
• Ghent University BE
• Hospital Universitari Germans Trias i Pujol ES
• Inserm FR
• Mount Allison University CA
• University of Birmingham GB
• The Ohio State University US
• Academic Center for Education, Culture and Research IR
• University of Auckland NZ
• Johns Hopkins University US
• Flemish Institute for Technological Research BE
• Centre for Immunity, Infection and Evolution GB

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