Multiplexed and portable nucleic acid detection platform with Cas13, Cas12a, and Csm6

Abstract

Taking CRISPR technology further CRISPR techniques are allowing the development of technologies for nucleic acid detection (see the Perspective by Chertow). Taking advantages of the distinctive enzymatic properties of CRISPR enzymes, Gootenberg et al. developed an improved nucleic acid detection technology for multiplexed quantitative and highly sensitive detection, combined with lateral flow for visual readout. Myhrvold et al. added a sample preparation protocol to create a field-deployable viral diagnostic platform for rapid detection of specific strains of pathogens in clinical samples. Cas12a (also known as Cpf1), a type V CRISPR protein, cleaves double-stranded DNA and has been adapted for genome editing. Chen et al. discovered that Cas12a also processes single-stranded DNA threading activity. A technology platform based on this activity detected human papillomavirus in patient samples with high sensitivity. Science , this issue p. 439 , p. 444 , p. 436 ; see also p. 381

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Affiliated Institutions

• Center for Systems Biology US
• Harvard University US
• IIT@MIT US
• Harvard–MIT Division of Health Sciences and Technology US
• Broad Institute US
• Massachusetts Institute of Technology US
• McGovern Institute for Brain Research US

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