Abstract

This image analysis algorithm provides a robust and flexible method for objective immunohistochemical analysis of samples stained with up to three different stains using a laboratory microscope, standard RGB camera setup and the public domain program NIH Image.

Keywords

StainStainingRGB color modelDeconvolutionEosinH&E stainArtificial intelligenceComputer sciencePathologyMedicineAlgorithm

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Publication Info

Year
2001
Type
article
Volume
23
Issue
4
Pages
291-9
Citations
2064
Access
Closed

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Arnout Ruifrok, D. A. Johnston (2001). Quantification of histochemical staining by color deconvolution.. PubMed , 23 (4) , 291-9.