Revised Method for Aflatoxins in Cottonseed Products, and Comparison of Thin Layer and High Performance Liquid Chromatography Determinative Steps: Collaborative Study

Walter A. Pons; Louise S. Lee; Leonard Stoloff; Albert K. Athnasios; R H Beebe; Olivier Francis; A. O. Franz; Joshua Routh; P M Scott; R Smiechowski; Robert D Stubblefield; J Theane; Charles W Thorpe; Mary W Trucksess; A E Waltking

doi:10.1093/jaoac/63.4.899

Abstract

Abstract Modifications to save analyst time and reagents have been made in the rapid method for separation and quantitation of aflatoxins in cottonseed products. A collaborative study of the revised method, including optional high performance liquid chromatography (HPLC) in addition to thin layer chromatography for the determinative step, showed no change in the method precision or accuracy due to the modifications and no significant difference in the values obtained by either determinative procedure. The HPLC technique provided no significant difference in analysis repeatability, but did reduce the betweenlaboratory error component. The method has been adopted as official first action.

Keywords

ChromatographyDeterminativeRepeatabilityHigh-performance liquid chromatographyChemistryThin-layer chromatographyAflatoxinReagentSignificant differenceMathematicsOrganic chemistryStatistics

Affiliated Institutions

Southern Regional Research Center US

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Publication Info

Year: 1980
Type: article
Volume: 63
Issue: 4
Pages: 899-906
Citations: 17
Access: Closed

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APA Style

                            
                                
                                    Walter A. Pons, 
                                
                                    Louise S. Lee, 
                                
                                    Leonard Stoloff
                                
                                et al.
                            
                            (1980). 
                            Revised Method for Aflatoxins in Cottonseed Products, and Comparison of Thin Layer and High Performance Liquid Chromatography Determinative Steps: Collaborative Study. 
                            Journal of AOAC INTERNATIONAL
                            , 63
                            (4)
                            , 899-906.
                            https://doi.org/10.1093/jaoac/63.4.899
                        

Identifiers

DOI: 10.1093/jaoac/63.4.899