Single-Cell RNA-Seq Reveals Dynamic, Random Monoallelic Gene Expression in Mammalian Cells

Qiaolin Deng; Daniel Ramsköld; Björn Reinius; Rickard Sandberg

doi:10.1126/science.1245316

Abstract

Expressing One Allele at a Time Although genetic traits are often dominant or recessive, the impact of the same heterozygous genotype can vary quite a bit between individuals. Deng et al. (p. 193 ), analyzed global gene expression in hundreds of individual mouse cells and found that a substantial fraction of the genes only expressed one of the alleles, chosen randomly, at any given point in time. Such stochasticity in transcription increases the heterogeneity among cells and likely contributes to the phenotypic variance among individuals of identical genotype.

Keywords

BiologyAlleleGeneticsGenotypeGenePhenotypeGene expressionRNAGenetic variationTranscription (linguistics)

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Publication Info

Year: 2014
Type: article
Volume: 343
Issue: 6167
Pages: 193-196
Citations: 1434
Access: Closed

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APA Style

                            
                                
                                    Qiaolin Deng, 
                                
                                    Daniel Ramsköld, 
                                
                                    Björn Reinius
                                
                                et al.
                            
                            (2014). 
                            Single-Cell RNA-Seq Reveals Dynamic, Random Monoallelic Gene Expression in Mammalian Cells. 
                            Science
                            , 343
                            (6167)
                            , 193-196.
                            https://doi.org/10.1126/science.1245316
                        

Identifiers

DOI: 10.1126/science.1245316