Targeting Cre Recombinase to Specific Neuron Populations with Bacterial Artificial Chromosome Constructs

Shiaoching Gong; Martin L. Doughty; Carroll R. Harbaugh; Alexander Cummins; Mary E. Hatten; Nathaniel Heintz; Charles R. Gerfen

doi:10.1523/jneurosci.2707-07.2007

Abstract

Transgenic mouse lines are characterized with Cre recombinase driven by promoters of CNS-specific genes using bacterial artificial chromosome (BAC) constructs. BAC-Cre constructs for 10 genes ( Chat , Th , Slc6a4 , Slc6a2 , Etv1 , Ntsr1 , Drd2 , Drd1 , Pcp2 , and Cmtm5 ) produced 14 lines with Cre expression in specific neuronal and glial populations in the brain. These Cre driver lines add functional utility to the >500 BAC-EGFP (enhanced green fluorescent protein) transgenic mouse lines that are part of the Gene Expression Nervous System Atlas Project.

Keywords

Bacterial artificial chromosomeCre recombinaseRecombinaseBiologyTransgeneGreen fluorescent proteinGeneGenetically modified mouseGeneticsChromosomeMolecular biologyCell biologyComputational biologyGenome

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Publication Info

Year: 2007
Type: article
Volume: 27
Issue: 37
Pages: 9817-9823
Citations: 1017
Access: Closed

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APA Style

                            
                                
                                    Shiaoching Gong, 
                                
                                    Martin L. Doughty, 
                                
                                    Carroll R. Harbaugh
                                
                                et al.
                            
                            (2007). 
                            Targeting Cre Recombinase to Specific Neuron Populations with Bacterial Artificial Chromosome Constructs. 
                            Journal of Neuroscience
                            , 27
                            (37)
                            , 9817-9823.
                            https://doi.org/10.1523/jneurosci.2707-07.2007
                        

Identifiers

DOI: 10.1523/jneurosci.2707-07.2007