The Power and Prospects of Fluorescence Microscopies and Spectroscopies

Abstract

▪ Abstract Recent years have witnessed a renaissance of fluorescence microscopy techniques and applications, from live-animal multiphoton confocal microscopy to single-molecule fluorescence spectroscopy and imaging in living cells. These achievements have been made possible not so much because of improvements in microscope design, but rather because of development of new detectors, accessible continuous wave and pulsed laser sources, sophisticated multiparameter analysis on one hand, and the development of new probes and labeling chemistries on the other. This review tracks the lineage of ideas and the evolution of thinking that have led to the actual developments, and presents a comprehensive overview of the field, with emphasis put on our laboratory's interest in single-molecule microscopy and spectroscopy.

Keywords

MicroscopyNanotechnologyFluorescence microscopeThe RenaissanceSuper-resolution microscopyMicroscopeMultiphoton fluorescence microscopeFluorescenceMaterials scienceOpticsPhysicsArt

MeSH Terms

Equipment DesignFluorescence Resonance Energy TransferFluorescent DyesMicroscopyFluorescenceSpectrometryFluorescenceStaining and Labeling

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Publication Info

Year: 2003
Type: review
Volume: 32
Issue: 1
Pages: 161-182
Citations: 213
Access: Closed

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APA Style

                            
                                
                                    Xavier Michalet, 
                                
                                    Achillefs N. Kapanidis, 
                                
                                    Ted A. Laurence
                                
                                et al.
                            
                            (2003). 
                            The Power and Prospects of Fluorescence Microscopies and Spectroscopies. 
                            Annual Review of Biophysics and Biomolecular Structure
                            , 32
                            (1)
                            , 161-182.
                            https://doi.org/10.1146/annurev.biophys.32.110601.142525
                        

Identifiers

DOI: 10.1146/annurev.biophys.32.110601.142525
PMID: 12598370

Data Quality

Data completeness: 81%