Use of pulsed-field gel electrophoresis for investigation of hospital outbreaks of Acinetobacter baumannii

A Gouby; M J Carles-Nurit; N. Bouziges; Gisèle Bourg; R. Mesnard; Philippe Bouvet

doi:10.1128/jcm.30.6.1588-1591.1992

Abstract

Genomic DNAs from taxonomically and epidemiologically well-defined strains of Acinetobacter baumannii were digested with restriction endonucleases that cleave with low frequency, and the fragments were separated by pulse-field gel electrophoresis. Restriction fragment length polymorphisms were observed. Restriction fragment length polymorphism analysis can be used as an epidemiological tool to delineate outbreaks of nosocomial infections caused by A. baumannii.

Keywords

Acinetobacter baumanniiRestriction fragment length polymorphismRestriction enzymePulsed-field gel electrophoresisOutbreakGel electrophoresisBiologyNeisseriaceaeMicrobiologyRestriction fragmentAcinetobacterAmplified fragment length polymorphismMolecular epidemiologyGenotypeVirologyGeneticsDNABacteriaGeneMedicinePseudomonas aeruginosaAntibioticsGenetic diversity

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Publication Info

Year: 1992
Type: article
Volume: 30
Issue: 6
Pages: 1588-1591
Citations: 118
Access: Closed

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APA Style

                            
                                
                                    A Gouby, 
                                
                                    M J Carles-Nurit, 
                                
                                    N. Bouziges
                                
                                et al.
                            
                            (1992). 
                            Use of pulsed-field gel electrophoresis for investigation of hospital outbreaks of Acinetobacter baumannii. 
                            Journal of Clinical Microbiology
                            , 30
                            (6)
                            , 1588-1591.
                            https://doi.org/10.1128/jcm.30.6.1588-1591.1992
                        

Identifiers

DOI: 10.1128/jcm.30.6.1588-1591.1992