Abstract

The use of semiconductor nanocrystals (quantum dots) as fluorescent labels for multiphoton microscopy enables multicolor imaging in demanding biological environments such as living tissue. We characterized water-soluble cadmium selenide–zinc sulfide quantum dots for multiphoton imaging in live animals. These fluorescent probes have two-photon action cross sections as high as 47,000 Goeppert-Mayer units, by far the largest of any label used in multiphoton microscopy. We visualized quantum dots dynamically through the skin of living mice, in capillaries hundreds of micrometers deep. We found no evidence of blinking (fluorescence intermittency) in solution on nanosecond to millisecond time scales.

Keywords

Quantum dotFluorescenceMultiphoton fluorescence microscopeMicroscopyNanosecondCadmium selenideFluorescence microscopeTwo-photon excitation microscopyFluorescence-lifetime imaging microscopyZinc selenideMaterials scienceOptoelectronicsChemistryNanotechnologyOpticsLaserPhysics

MeSH Terms

Adipose TissueAnimalsBlood Flow VelocityCadmium CompoundsCapillariesCrystallizationDextransDiagnostic ImagingFluorescein-5-isothiocyanateFluorescenceFluorescent DyesMiceMicroscopyNanotechnologyPhotonsSelenium CompoundsSemiconductorsSkinSolubilitySpectrometryFluorescenceSulfidesWaterZinc Compounds

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Publication Info

Year
2003
Type
article
Volume
300
Issue
5624
Pages
1434-1436
Citations
2263
Access
Closed

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Cite This

Daniel R. Larson, Warren R. Zipfel, Rebecca M. Williams et al. (2003). Water-Soluble Quantum Dots for Multiphoton Fluorescence Imaging in Vivo. Science , 300 (5624) , 1434-1436. https://doi.org/10.1126/science.1083780

Identifiers

DOI
10.1126/science.1083780
PMID
12775841

Data Quality

Data completeness: 81%