J. Craig Venter

Enzymatic assembly of DNA molecules up to several hundred kilobases

Complete Chemical Synthesis, Assembly, and Cloning of a <i>Mycoplasma genitalium</i> Genome

We have synthesized a 582,970–base pair Mycoplasma genitalium genome. This synthetic genome, named M. genitalium JCVI-1.0, contains all the genes of wild-type M. genitalium G37 ...

Cell-free cloning using φ29 DNA polymerase

We describe conditions for rolling-circle amplification (RCA) of individual DNA molecules 5–7 kb in size by &gt;10 9 -fold, using φ29 DNA polymerase. The principal difficulty wi...

The Sequence of the Human Genome

A 2.91-billion base pair (bp) consensus sequence of the euchromatic portion of the human genome was generated by the whole-genome shotgun sequencing method. The 14.8-billion bp ...

A Whole-Genome Assembly of <i>Drosophila</i>

We report on the quality of a whole-genome assembly of Drosophila melanogaster and the nature of the computer algorithms that accomplished it. Three independent external data so...

Complete Genome Sequence of a Virulent Isolate of <i>Streptococcus pneumoniae</i>

The 2,160,837–base pair genome sequence of an isolate of Streptococcus pneumoniae , a Gram-positive pathogen that causes pneumonia, bacteremia, meningitis, and otitis media, con...

Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana

Comparative Genomics of the Eukaryotes

A comparative analysis of the genomes of Drosophila melanogaster , Caenorhabditis elegans , and Saccharomyces cerevisiae —and the proteins they are predicted to encode—was under...

Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana

The Sorcerer II Global Ocean Sampling Expedition: Northwest Atlantic through Eastern Tropical Pacific

The world's oceans contain a complex mixture of micro-organisms that are for the most part, uncharacterized both genetically and biochemically. We report here a metagenomic stud...

The Sorcerer II Global Ocean Sampling Expedition: Expanding the Universe of Protein Families

Metagenomics projects based on shotgun sequencing of populations of micro-organisms yield insight into protein families. We used sequence similarity clustering to explore protei...

Complementary DNA Sequencing: Expressed Sequence Tags and Human Genome Project

Automated partial DNA sequencing was conducted on more than 600 randomly selected human brain complementary DNA (cDNA) clones to generate expressed sequence tags (ESTs). ESTs ha...

The Diploid Genome Sequence of an Individual Human

Presented here is a genome sequence of an individual human. It was produced from approximately 32 million random DNA fragments, sequenced by Sanger dideoxy technology and assemb...