Kevin Struhl

[35] The use of random-sequence oligonucleotides for determining consensus sequences

Defining the Sequence Specificity of DNA-Binding Proteins by Selecting Binding Sites from Random-Sequence Oligonucleotides: Analysis of Yeast GCN4 Protein

We describe a new method for accurately defining the sequence recognition properties of DNA-binding proteins by selecting high-affinity binding sites from random-sequence DNA. T...

Defining the consensus sequences of<i>E.coli</i>promoter elements by random selectio

The consensus sequence of E.coli promoter elements was determined by the method of random selection. A large collection of hybrid molecules was produced in which random-sequence...

Nucleotide sequence and transcriptional mapping of the yeast<i>pet56-his3-dedl</i>gene region

Genes of the baker's yeast Saccharomyces cerevisiae are densely clustered on 16 linear chromosomes. Here, I characterize a 1.8 kb region of chromosome XV containing the entire s...

Two related regulatory sequences are required for maximal induction of Saccharomyces cerevisiae his3 transcription.

In Saccharomyces cerevisiae, the coordinate induction of his3 and other amino acid biosynthesis genes is mediated by the binding of GCN4 activator protein to specific promoter s...

Histone acetylation and transcriptional regulatory mechanisms

More than 30 years ago, Vincent Allfrey proposed that histone acetylation was associated with transcriptional activity in eukaryotic cells (Allfrey et al. 1964; Pogo et al. 1966...