A gene expression atlas of the central nervous system based on bacterial artificial chromosomes

Shiaoching Gong; Zheng Chen; Martin L. Doughty; Kasia Losos; Nicholas Didkovsky; Uta B. Schambra; Norma J. Nowak; Alexandra L. Joyner; Gabrielle G. Leblanc; Mary E. Hatten; Nathaniel Heintz

doi:10.1038/nature02033

Abstract

The mammalian central nervous system (CNS) contains a remarkable array of neural cells, each with a complex pattern of connections that together generate perceptions and higher brain functions. Here we describe a large-scale screen to create an atlas of CNS gene expression at the cellular level, and to provide a library of verified bacterial artificial chromosome (BAC) vectors and transgenic mouse lines that offer experimental access to CNS regions, cell classes and pathways. We illustrate the use of this atlas to derive novel insights into gene function in neural cells, and into principal steps of CNS development. The atlas, library of BAC vectors and BAC transgenic mice generated in this screen provide a rich resource that allows a broad array of investigations not previously available to the neuroscience community.

Keywords

Bacterial artificial chromosomeAtlas (anatomy)BiologyBrain atlasHuman artificial chromosomeGeneComputational biologyCentral nervous systemTransgeneNeuroscienceGenomeGeneticsChromosomeAnatomy

MeSH Terms

AnimalsAxonsCell DifferentiationCell LineageCell MovementCentral Nervous SystemChromosomesArtificialBacterialCyclic Nucleotide PhosphodiesterasesType 1Gene Expression ProfilingGene Expression RegulationDevelopmentalGene LibraryGenesReporterGenetic VectorsGreen Fluorescent ProteinsHomeodomain ProteinsLIM-Homeodomain ProteinsLuminescent ProteinsMembrane GlycoproteinsMiceMiceTransgenicNerve Tissue ProteinsNeurosciencesPhosphoric Diester HydrolasesSynapsesTranscription FactorsTransgenes

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Publication Info

Year: 2003
Type: article
Volume: 425
Issue: 6961
Pages: 917-925
Citations: 2130
Access: Closed

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APA Style

                            
                                
                                    Shiaoching Gong, 
                                
                                    Zheng Chen, 
                                
                                    Martin L. Doughty
                                
                                et al.
                            
                            (2003). 
                            A gene expression atlas of the central nervous system based on bacterial artificial chromosomes. 
                            Nature
                            , 425
                            (6961)
                            , 917-925.
                            https://doi.org/10.1038/nature02033
                        

Identifiers

DOI: 10.1038/nature02033
PMID: 14586460

Data Quality

Data completeness: 81%