Abstract
Abstract The 3 rapid screening methods studied all involved chromatography of partially purified extracts on minicolumns. In one method acetonitrile-water is used to extract aflatoxin from corn and a lead acetate precipitation removes impurities from the extracts. The second method specifies acetone-water as the extracting solvent and ammonium sulfate as the precipitant. The third method is based on acetone-water as the extracting solvent and ferric gel as the precipitant. The most sensitive method was the ferric gel precipitation, which could detect 5 ppb aflatoxin, but it was very time-consuming. The most satisfactory method (timewise) used the ammonium sulfate and had a sensitivity of 10 ppb. This method also led to the best differentiation between levels of aflatoxin. These 2 methods have been adopted as official first action.
Keywords
AflatoxinAcetoneChemistrySolventChromatographyAmmonium acetateAmmonium sulfateEthyl acetatePrecipitationAcetonitrileFerricProtein precipitationAmmoniumExtraction (chemistry)High-performance liquid chromatographyInorganic chemistryOrganic chemistryFood science
Affiliated Institutions
Related Publications
Modified Rapid Screening Method for Aflatoxin in Corn
Abstract A rapid screening method for detecting aflatoxin in cottonseed products was investigated to determine if it was applicable to corn. Other aflatoxin-extracting solvents ...
Improved Cleanup for Liquid Chromatographic Analysis and Fluorescence Detection of Aflatoxins M1 and M2 in Fluid Milk Products
Abstract A rapid method is described for extraction and cleanup of raw and processed milk for determination of anatoxins M1 and M2 by using a C18 Sep-Pak/silica gel cleanup colu...
Gas Chromatographic Method for Ethylene Dibromide in Grains and Grain-Based Products: Collaborative Study
Abstract Nine laboratories analyzed samples of whole grain, intermediate, and ready-to-eat products for ethylene dibromide (EDB) residues. Supplied samples of wheat, rice, and f...
Collaborative Study of Methods for the Determination and Chemical Confirmation of Aflatoxin M1 in Dairy Products
Abstract An international collaborative study involving 19 collaborators was conducted to test methods for the determination and chemical confirmation of aflatoxin M1 in dairy p...
Enzymatic polymerization: The first in vitro synthesis of cellulose via nonbiosynthetic path catalyzed by cellulase
Abstract The in vitro synthesis of cellulose via a nonbiosynthetic path has been achieved for the first time by condensation of β‐D‐cellobiosyl fluoride as substrate for cellula...
Publication Info
- Year
- 1973
- Type
- article
- Volume
- 56
- Issue
- 4
- Pages
- 808-812
- Citations
- 3
- Access
- Closed
External Links
Social Impact
Altmetric
PlumX Metrics
Social media, news, blog, policy document mentions
Citation Metrics
3
OpenAlex
Cite This
Odette L Shotwell,
Robert D Stubblefield
(1973).
Collaborative Study of Three Screening Methods for Aflatoxin in Corn.
Journal of AOAC INTERNATIONAL
, 56
(4)
, 808-812.
https://doi.org/10.1093/jaoac/56.4.808
Identifiers
- DOI
- 10.1093/jaoac/56.4.808