Abstract
Genomic DNAs of Escherichia coli O157:H7 strains isolated from patients and food samples were analyzed by pulsed-field gel electrophoresis. The rare-cutting endonucleases SfiI and XbaI generated 6 and 10 distinct genomic profiles, respectively, for the 22 strains analyzed, indicating that this technique may find application for epidemiologic studies. Summation of XbaI fragments from five E. coli O157:H7 strains estimated the genomic length at ca. 4.7 Mb.
Keywords
Escherichia coliPulsed-field gel electrophoresisGel electrophoresisBiologygenomic DNARestriction enzymeGeneticsMolecular biologyElectrophoresisDNAGenotypeGene
Affiliated Institutions
Related Publications
Genome size and restriction fragment length polymorphism analysis of<i>Vibrio cholerae</i>strains belonging to different serovars and biotypes
The genome size of Vibrio cholerae has been determined by pulsed field gel electrophoresis following digestion of chromosomal DNA with endonucleases. The genome size of all the ...
Pulsed-Field Gel Electrophoresis of Campylobacter jejuni and Campylobacter coli Genomic DNA and Its Epidemiologic Application
Genomic DNA from 12 different Campylobacter jejuni and 10 Campylobacter coli isolates was digested with SmaI and analyzed by pulsed-field gel electrophoresis (PFGE) and 16S rRNA...
DNA fingerprinting of Streptococcus pneumoniae strains by pulsed-field gel electrophoresis
Pulsed-field gel electrophoresis of genomic DNA was carried out on Streptococcus pneumoniae strains to determine its value in the epidemiological survey of pneumococcal infectio...
Use of low-frequency-cleavage restriction endonucleases for DNA analysis in epidemiological investigations of nosocomial bacterial infections
Epidemiological investigations of bacterial infections are generally based on multiple phenotypic markers that are often difficult to verify. A more general and reliable method ...
Use of pulsed-field gel electrophoresis for investigation of hospital outbreaks of Acinetobacter baumannii
Genomic DNAs from taxonomically and epidemiologically well-defined strains of Acinetobacter baumannii were digested with restriction endonucleases that cleave with low frequency...
Publication Info
- Year
- 1993
- Type
- article
- Volume
- 59
- Issue
- 9
- Pages
- 3141-3144
- Citations
- 55
- Access
- Closed
External Links
Social Impact
Altmetric
PlumX Metrics
Social media, news, blog, policy document mentions
Citation Metrics
55
OpenAlex
Cite This
K D Harsono,
Charles W. Kaspar,
John B. Luchansky
(1993).
Comparison and genomic sizing of Escherichia coli O157:H7 isolates by pulsed-field gel electrophoresis.
Applied and Environmental Microbiology
, 59
(9)
, 3141-3144.
https://doi.org/10.1128/aem.59.9.3141-3144.1993
Identifiers
- DOI
- 10.1128/aem.59.9.3141-3144.1993