Abstract
Plasmid DNA directing transcription of the noncoding (anti-sense) DNA strand can specifically inhibit the expression of several test genes as well as normal, endogenous genes. The anti-sense plasmid constructions can be introduced into eukaryotic cells by transfection or microinjection and function in both transient and stable transformation assays. Anti-sense transcripts complementary to as little as 52 bases of 5′ untranslated target gene mRNA specifically suppress gene activity as well as, or more efficiently than, anti-sense transcripts directed against the protein coding domain alone. Conditional anti-sense inhibition is accomplished with the use of hormone-inducible promoter sequences. Suppression of endogenous actin gene activity by anti-sense RNA is detected as a decrease in growth rate and as a reduction in the number of actin microfilament cables. These observations suggest that anti-sense RNA may be generally useful for suppressing the expression of specific genes in vivo and may be a potential molecular alternative to classical genetic analysis.
Keywords
Sense (electronics)GeneBiologyAntisense RNARNAPlasmidGene expressionTranscription (linguistics)GeneticsDNAMolecular biologyCell biologyChemistry
MeSH Terms
AcetyltransferasesActinsAnimalsCattleChickensChloramphenicol O-AcetyltransferaseDNARecombinantDrosophilaGenesGenesViralGenetic EngineeringGenetic VectorsGlobinsPlasmidsRNAMessengerSimplexvirusSuppressionGeneticThymidine KinaseXenopus
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Publication Info
- Year
- 1985
- Type
- article
- Volume
- 229
- Issue
- 4711
- Pages
- 345-352
- Citations
- 343
- Access
- Closed
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Cite This
Jonathan G. Izant,
Harold Weintraub
(1985).
Constitutive and Conditional Suppression of Exogenous and Endogenous Genes by Anti-Sense RNA.
Science
, 229
(4711)
, 345-352.
https://doi.org/10.1126/science.2990048
Identifiers
- DOI
- 10.1126/science.2990048
- PMID
- 2990048