Finished bacterial genomes from shotgun sequence data

Abstract

Exceptionally accurate genome reference sequences have proven to be of great value to microbial researchers. Thus, to date, about 1800 bacterial genome assemblies have been “finished” at great expense with the aid of manual laboratory and computational processes that typically iterate over a period of months or even years. By applying a new laboratory design and new assembly algorithm to 16 samples, we demonstrate that assemblies exceeding finished quality can be obtained from whole-genome shotgun data and automated computation. Cost and time requirements are thus dramatically reduced.

Keywords

BiologyShotgunShotgun sequencingComputational biologyGenomeGeneticsSequence (biology)Whole genome sequencingGene

Affiliated Institutions

Broad Institute US

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Publication Info

Year: 2012
Type: article
Volume: 22
Issue: 11
Pages: 2270-2277
Citations: 219
Access: Closed

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APA Style

                            
                                
                                    Filipe J. Ribeiro, 
                                
                                    Dariusz Przybylski, 
                                
                                    Shuangye Yin
                                
                                et al.
                            
                            (2012). 
                            Finished bacterial genomes from shotgun sequence data. 
                            Genome Research
                            , 22
                            (11)
                            , 2270-2277.
                            https://doi.org/10.1101/gr.141515.112
                        

Identifiers

DOI: 10.1101/gr.141515.112