Functional Characterization of the S. cerevisiae Genome by Gene Deletion and Parallel Analysis

Elizabeth A. Winzeler; Daniel Shoemaker; Anna Astromoff; Hong Liang; Keith M. Anderson; Bruno André; Rhonda Bangham; Rocí­o Benito; Jef D. Boeke; Howard Bussey; Angela Chu; Carla J. Connelly; Karen Davis; Fred S. Dietrich; Sally Dow; Mohamed El Bakkoury; Françoise Foury; Stephen Friend; Erik Gentalen; Guri Giaever; Johannes H. Hegemann; Ted Jones; Michael T. Laub; Hong Liao; Nicole Liebundguth; David J. Lockhart; Anca Lucau‐Danila; Marc Lussier; Nasiha M’Rabet; P Ménard; Michael Mittmann; Chai Pai; Corinne Rebischung; José Luis Revuelta; Linda Riles; Christopher J. Roberts; Petra Ross‐Macdonald; Bart Scherens; M Snyder; Sharon Sookhai-Mahadeo; Reginald Storms; Steeve Véronneau; Marleen Voet; Guido Volckaert; Teresa R. Ward; Robert Wysocki; Grace S. Yen; Kexin Yu; Katja Zimmermann; Peter Philippsen; Mark Johnston; Ronald W. Davis

doi:10.1126/science.285.5429.901

Abstract

The functions of many open reading frames (ORFs) identified in genome-sequencing projects are unknown. New, whole-genome approaches are required to systematically determine their function. A total of 6925 Saccharomyces cerevisiae strains were constructed, by a high-throughput strategy, each with a precise deletion of one of 2026 ORFs (more than one-third of the ORFs in the genome). Of the deleted ORFs, 17 percent were essential for viability in rich medium. The phenotypes of more than 500 deletion strains were assayed in parallel. Of the deletion strains, 40 percent showed quantitative growth defects in either rich or minimal medium.

Keywords

ORFSGenomeBiologyOpen reading frameGeneticsSaccharomyces cerevisiaeGeneWhole genome sequencingPhenotypeFunction (biology)Genome sizeComputational biologyPeptide sequence

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Publication Info

Year: 1999
Type: article
Volume: 285
Issue: 5429
Pages: 901-906
Citations: 3977
Access: Closed

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APA Style

                            
                                
                                    Elizabeth A. Winzeler, 
                                
                                    Daniel Shoemaker, 
                                
                                    Anna Astromoff
                                
                                et al.
                            
                            (1999). 
                            Functional Characterization of the <i>S. cerevisiae</i> Genome by Gene Deletion and Parallel Analysis. 
                            Science
                            , 285
                            (5429)
                            , 901-906.
                            https://doi.org/10.1126/science.285.5429.901
                        

Identifiers

DOI: 10.1126/science.285.5429.901