Abstract
Adaptive responses to hypoxia occur in many biological systems. A well-characterized example is the hypoxic induction of the synthesis of erythropoietin, a hormone which regulates erythropoiesis and hence blood oxygen content. The restricted expression of the erythropoietin gene in subsets of cells within kidney and liver has suggested that this specific oxygen-sensing mechanism is restricted to specialized cells in those organs. Using transient transfection of reporter genes coupled to a transcriptional enhancer lying 3' to the erythropoietin gene, we show that an oxygen-sensing system similar, or identical, to that controlling erythropoietin expression is wide-spread in mammalian cells. The extensive distribution of this sensing mechanism contrasts with the restricted expression of erythropoietin, suggesting that it mediates other adaptive responses to hypoxia.
Keywords
ErythropoietinErythropoiesisEnhancerBiologyTransfectionCell biologyHypoxia (environmental)Mechanism (biology)Cell cultureGeneGene expressionOxygenChemistryEndocrinologyInternal medicineBiochemistryGeneticsAnemiaMedicine
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Publication Info
- Year
- 1993
- Type
- article
- Volume
- 90
- Issue
- 6
- Pages
- 2423-2427
- Citations
- 413
- Access
- Closed
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Cite This
P H Maxwell,
Christopher W. Pugh,
Peter J. Ratcliffe
(1993).
Inducible operation of the erythropoietin 3' enhancer in multiple cell lines: evidence for a widespread oxygen-sensing mechanism..
Proceedings of the National Academy of Sciences
, 90
(6)
, 2423-2427.
https://doi.org/10.1073/pnas.90.6.2423
Identifiers
- DOI
- 10.1073/pnas.90.6.2423