Abstract
Hypoxia-inducible factor 1 (HIF-1) is found in mammalian cells cultured under reduced O2 tension and is necessary for transcriptional activation mediated by the erythropoietin gene enhancer in hypoxic cells. We show that both HIF-1 subunits are basic-helix-loop-helix proteins containing a PAS domain, defined by its presence in the Drosophila Per and Sim proteins and in the mammalian ARNT and AHR proteins. HIF-1 alpha is most closely related to Sim. HIF-1 beta is a series of ARNT gene products, which can thus heterodimerize with either HIF-1 alpha or AHR. HIF-1 alpha and HIF-1 beta (ARNT) RNA and protein levels were induced in cells exposed to 1% O2 and decayed rapidly upon return of the cells to 20% O2, consistent with the role of HIF-1 as a mediator of transcriptional responses to hypoxia.
Keywords
Aryl hydrocarbon receptor nuclear translocatorBasic helix-loop-helixPAS domainBiologyEnhancerHypoxia-Inducible Factor 1Cell biologyMolecular biologyG alpha subunitTranscription factorMediatorAlpha (finance)Alpha helixGeneChemistryProtein structureBiochemistryDNA-binding proteinProtein subunitMedicine
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Publication Info
- Year
- 1995
- Type
- article
- Volume
- 92
- Issue
- 12
- Pages
- 5510-5514
- Citations
- 6119
- Access
- Closed
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Cite This
G L Wang,
Bing‐Hua Jiang,
E A Rue
et al.
(1995).
Hypoxia-inducible factor 1 is a basic-helix-loop-helix-PAS heterodimer regulated by cellular O2 tension..
Proceedings of the National Academy of Sciences
, 92
(12)
, 5510-5514.
https://doi.org/10.1073/pnas.92.12.5510
Identifiers
- DOI
- 10.1073/pnas.92.12.5510