Abstract

Quantum dots having four different surface coatings were tested for use in in vivo imaging. Localization was successfully monitored by fluorescence imaging of living animals, by necropsy, by frozen tissue sections for optical microscopy, and by electron microscopy, on scales ranging from centimeters to nanometers, using only quantum dots for detection. Circulating half-lives were found to be less than 12 min for amphiphilic poly(acrylic acid), short-chain (750 Da) methoxy-PEG or long-chain (3400 Da) carboxy-PEG quantum dots, but approximately 70 min for long-chain (5000 Da) methoxy-PEG quantum dots. Surface coatings also determined the in vivo localization of the quantum dots. Long-term experiments demonstrated that these quantum dots remain fluorescent after at least four months in vivo.

Keywords

Quantum dotChemistryIn vivoFluorescenceFluorescence microscopeMicroscopyNanotechnologyElectron microscopePEG ratioNanometreBiophysicsOpticsMaterials sciencePhysics

MeSH Terms

AnimalsDiagnostic ImagingFluorescenceFluorescent DyesImage ProcessingComputer-AssistedMiceMiceInbred BALB CMiceNudeMicroscopyElectronMicroscopyFluorescencePolyethylene GlycolsSpleen

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Publication Info

Year
2003
Type
article
Volume
15
Issue
1
Pages
79-86
Citations
1125
Access
Closed

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Cite This

Byron Ballou, B. Christoffer Lagerholm, Lauren A. Ernst et al. (2003). Noninvasive Imaging of Quantum Dots in Mice. Bioconjugate Chemistry , 15 (1) , 79-86. https://doi.org/10.1021/bc034153y

Identifiers

DOI
10.1021/bc034153y
PMID
14733586

Data Quality

Data completeness: 86%