Studies in humanized mice and convalescent humans yield a SARS-CoV-2 antibody cocktail

Johanna K. Hansen , Alina Baum , Kristen E. Pascal , Johanna K. Hansen , Alina Baum , Kristen E. Pascal , Vincenzo Russo , Stephanie Giordano , Elzbieta Wloga , Benjamin O. Fulton , Ying Yan , Katrina Koon , Krunal Patel , Kyung Min Chung , Aynur Hermann , Erica Ullman , Jonathan W. Cruz , Ashique Rafique , Tammy Huang , Jeanette Fairhurst , Christen Libertiny , Marine Malbec , Wen‐Yi Lee , Richard S. Welsh , Glen A. Farr , Seth Pennington , Dipali Deshpande , Jemmie Cheng , Anke Watty , Pascal Bouffard , Robert Babb , Natasha Levenkova , Calvin Chen , Bojie Zhang , Annabel Romero Hernandez , Kei Saotome , Yi Zhou , Matthew C. Franklin , Sumathi Sivapalasingam , David Chien Lye , Stuart Weston , James Logue , Robert Haupt , Matthew B. Frieman , Gang Chen , William C. Olson , Andrew Murphy , Neil Stahl , George D. Yancopoulos , Christos A. Kyratsous
2020 Science 1,398 citations

Abstract

An antibody cocktail against SARS-CoV-2 There is an urgent focus on antibodies that target the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) viral spike and prevent the virus from entering host cells. Hansen et al. generated a large panel of antibodies against the spike protein from humanized mice and recovered patients. From this panel, they identified several neutralizing antibodies, including pairs that do not compete for binding to the receptor binding domain. Baum et al. focused in on four of these antibodies. All four are effective against known spike variants. However, by growing a pseudovirus that expresses the spike in the presence of individual antibodies, the authors were able to select for spike mutants resistant to that antibody. In contrast, escape mutants are not selected when pseudovirus is grown in the presence of pairs of antibodies that either do not compete or only partially compete for binding to the RBD. Such a pair might be used in a therapeutic antibody cocktail. Science , this issue p. 1010 , p. 1014

Keywords

AntibodyHumanized antibodyConvalescent plasmaNeutralizationVirologyHumanized mouseSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2)Spike ProteinNeutralizing antibodyCoronavirusCoronavirus disease 2019 (COVID-19)Severe acute respiratory syndrome coronavirusRecombinant DNAImmunologyBiologyMedicineGeneMonoclonal antibodyImmune systemInfectious disease (medical specialty)GeneticsDisease

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Year
2020
Type
article
Volume
369
Issue
6506
Pages
1010-1014
Citations
1398
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Johanna K. Hansen, Alina Baum, Kristen E. Pascal et al. (2020). Studies in humanized mice and convalescent humans yield a SARS-CoV-2 antibody cocktail. Science , 369 (6506) , 1010-1014. https://doi.org/10.1126/science.abd0827

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DOI
10.1126/science.abd0827