Subcellular localization of the bcl-2 protein in malignant and normal lymphoid cells.

Daphne de Jong; F. Prins; D Y Mason; John C. Reed; G B van Ommen; P. M. Kluin

Abstract

The bcl-2 oncogene is expressed in lymphoid and myeloid cells as well as in neurons and several types of epithelial cells and inhibits programmed cell death (apoptosis). Deregulation by the t(14;18) translocation in lymphoid malignancies induces inappropriate cell survival and serves as one of the steps toward a fully malignant behavior. Using pre- and postembedding immunoelectron microscopy in normal and neoplastic lymphocytes, we demonstrate bcl-2 immunoreactivity to the mitochondrial outer circumference and the nuclear envelope and to a lesser degree to the cell membrane. Mitochondrial staining was patchy, reminiscent of mitochondrial contact zones. Additionally, there was a suggestion of association with nuclear pores. In these regions, transmembrane transport is mediated. This may suggest that bcl-2 exerts its function in this process.

Keywords

Immunoelectron microscopyBiologyCell biologyMyeloidMitochondrionTransmembrane proteinApoptosisOncogeneCellProgrammed cell deathPathologyLymphopoiesisCancer researchHaematopoiesisImmunohistochemistryImmunologyCell cycleReceptorBiochemistryMedicine

Affiliated Institutions

Leiden University NL

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Publication Info

Year: 1994
Type: article
Volume: 54
Issue: 1
Pages: 256-60
Citations: 191
Access: Closed

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APA Style

                            
                                    Daphne de Jong, 
                                
                                    F. Prins, 
                                
                                    D Y Mason
                                
                                et al.
                            
                            (1994). 
                            Subcellular localization of the bcl-2 protein in malignant and normal lymphoid cells.. 
                            PubMed
                            , 54
                            (1)
                            , 256-60.