Systematic search for zinc‐binding proteins in Escherichia coli

Abstract

A systematic search for Escherichia coli proteins with the zinc‐binding activity was performed using the assay of radioactive Zn(II) binding to total E. coli proteins fractionated by two methods of two‐dimensional gel electrophoresis. A total of 30–40 radioactive spots were identified, of which 14 have been assigned from N‐terminal sequencing. In addition to five known zinc‐binding proteins, nine zinc‐binding proteins were newly identified including: acetate kinase (AckA), DnaK, serine hydroxymethyltransferase (GlyA), transketolase isozymes (TktA/TktB), translation elongation factor Ts (Tsf), ribosomal proteins L2 (RplB), L13 (RplM) and one of S15 (RpsO), S16 (RpsP) or S17 (RpsQ). Together with about 20 known zinc‐binding proteins, the total number of zinc‐binding proteins in E. coli increased up to more than 30 species (or more than 3% of about 1000 proteins expressed under laboratory culture conditions). The specificity and affinity of zinc‐binding were analysed for some of the zinc‐binding proteins.

Keywords

Escherichia coliZincRibosomal proteinBiochemistryBiologyDNA-binding proteinBinding proteinBinding siteGel electrophoresisMolecular biologyChemistryRibosomeGeneTranscription factorRNA

MeSH Terms

Bacterial ProteinsCarrier ProteinsEscherichia coliZinc

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Publication Info

Year: 2002
Type: article
Volume: 269
Issue: 9
Pages: 2403-2413
Citations: 65
Access: Closed

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APA Style

                            
                                
                                    Akira Katayama, 
                                
                                    Atsuko Tsujii, 
                                
                                    Akira Wada
                                
                                et al.
                            
                            (2002). 
                            Systematic search for zinc‐binding proteins in <i>Escherichia coli</i>. 
                            European Journal of Biochemistry
                            , 269
                            (9)
                            , 2403-2413.
                            https://doi.org/10.1046/j.1432-1033.2002.02900.x
                        

Identifiers

DOI: 10.1046/j.1432-1033.2002.02900.x
PMID: 11985624

Data Quality

Data completeness: 81%

Systematic search for zinc‐binding proteins in <i>Escherichia coli</i>