Abstract
A telomere terminal transferase activity was identified in developing macronuclear extracts from Euplotes crassus. The activity was essentially unregulated in vitro: up to 50 tandem repeats of the Euplotes telomeric repeat sequence TTTTGGGG were added onto synthetic telomeric oligonucleotide primers. Both the structure of the telomere substrate and its 3'-terminal sequence were recognized. The activity was destroyed by low concentrations of RNase A.
Keywords
BiologyTelomereTandem repeatGeneticsOligonucleotideMolecular biologyRNase PLong terminal repeatDNAGeneGenomeRNA
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Publication Info
- Year
- 1989
- Type
- article
- Volume
- 9
- Issue
- 6
- Pages
- 2761-2764
- Citations
- 116
- Access
- Closed
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Cite This
Dorothy Shippen-Lentz,
Elizabeth H. Blackburn
(1989).
Telomere terminal transferase activity from Euplotes crassus adds large numbers of TTTTGGGG repeats onto telomeric primers..
Molecular and Cellular Biology
, 9
(6)
, 2761-2764.
https://doi.org/10.1128/mcb.9.6.2761
Identifiers
- DOI
- 10.1128/mcb.9.6.2761