Towards construction of a high resolution map of the mouse genome using PCR-analysed microsatellites

Jennifer M. Love; Andrew M. Knight; Marcia A. McAleer; John A. Todd

doi:10.1093/nar/18.14.4123

Abstract

Fifty sequences from the mouse genome database containing simple sequence repeats or microsatellites have been analysed for size variation using the polymerase chain reaction and gel electrophoresis. 88% of the sequences, most of which contain the dinucleotide repeat, CA/GT, showed size variations between different inbred strains of mice and the wild mouse, Mus spretus. 62% of sequences had 3 or more alleles. GA/CT and AT/TA-containing sequences were also variable. About half of these size variants were detectable by agarose gel electrophoresis. This simple approach is extremely useful in linkage and genome mapping studies and will facilitate construction of high resolution maps of both the mouse and human genomes.

Keywords

BiologyMicrosatelliteGenomeGeneticsComputational biologyPolymerase chain reactionHigh resolutionGene mappingEvolutionary biologyGeneRemote sensingChromosomeAllele

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Publication Info

Year: 1990
Type: article
Volume: 18
Issue: 14
Pages: 4123-4130
Citations: 468
Access: Closed

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APA Style

                            
                                
                                    Jennifer M. Love, 
                                
                                    Andrew M. Knight, 
                                
                                    Marcia A. McAleer
                                
                                et al.
                            
                            (1990). 
                            Towards construction of a high resolution map of the mouse genome using PCR-analysed microsatellites. 
                            Nucleic Acids Research
                            , 18
                            (14)
                            , 4123-4130.
                            https://doi.org/10.1093/nar/18.14.4123
                        

Identifiers

DOI: 10.1093/nar/18.14.4123