Keywords
NitrocelluloseSodium dodecyl sulfateBlotChemistryPolyacrylamideChromatographyPolyacrylamide gel electrophoresisElectrophoresisSodiumGel electrophoresisAntibodyBiochemistryBiologyEnzymeMembranePolymer chemistry
Affiliated Institutions
Related Publications
Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.
A simple and rapid method for transferring RNA from agarose gels to nitrocellulose paper for blot hybridization has been developed. Poly(A)+ and ribosomal RNAs transfer efficien...
Rapid and sensitive colorimetric method for visualizing biotin-labeled DNA probes hybridized to DNA or RNA immobilized on nitrocellulose: Bio-blots.
Biotin-labelled DNA probes, prepared by nick-translation in the presence of biotinylated analogs of TTP, are hybridized to DNA or RNA immobilized on nitrocellulose filters. Afte...
Publication Info
- Year
- 1981
- Type
- article
- Volume
- 112
- Issue
- 2
- Pages
- 195-203
- Citations
- 8470
- Access
- Closed
External Links
Social Impact
Altmetric
PlumX Metrics
Social media, news, blog, policy document mentions
Citation Metrics
8470
OpenAlex
Cite This
W. Neal Burnette
(1981).
“Western Blotting”: Electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A.
Analytical Biochemistry
, 112
(2)
, 195-203.
https://doi.org/10.1016/0003-2697(81)90281-5
Identifiers
- DOI
- 10.1016/0003-2697(81)90281-5