Calvin B. Harley

Telomeres shorten during ageing of human fibroblasts

Telomere length predicts replicative capacity of human fibroblasts.

When human fibroblasts from different donors are grown in vitro, only a small fraction of the variation in their finite replicative capacity is explained by the chronological ag...

Telomere shortening associated with chromosome instability is arrested in immortal cells which express telomerase activity.

Evidence for a mitotic clock in human hematopoietic stem cells: loss of telomeric DNA with age.

The proliferative life-span of the stem cells that sustain hematopoiesis throughout life is not known. It has been proposed that the sequential loss of telomeric DNA from the en...

Specific Association of Human Telomerase Activity with Immortal Cells and Cancer

Synthesis of DNA at chromosome ends by telomerase may be necessary for indefinite proliferation of human cells. A highly sensitive assay for measuring telomerase activity was de...

Telomerase activity in human ovarian carcinoma.

Telomeres fulfill the dual function of protecting eukaryotic chromosomes from illegitimate recombination and degradation and may aid in chromosome attachment to the nuclear memb...

Telomerase, Cell Immortality, and Cancer

An important hallmark of cancer is aberrant growth control. Genetic changes that confer a growth advantage to the tumor cell are observed on numerous levels. Some of the best un...

Stabilization of short telomeres and telomerase activity accompany immortalization of Epstein-Barr virus-transformed human B lymphocytes

We have measured telomere length and telomerase activity throughout the life span of clones of human B lymphocytes transformed by Epstein-Barr virus. Shortening of telomeres occ...

Loss of repetitious DNA in proliferating somatic cells may be due to unequal recombination