Abstract
Whether a single major histocompatibility complex (MHC)–bound peptide can drive the positive selection of large numbers of T cells has been a controversial issue. A diverse population of self peptides was shown to be essential for the in vivo development of CD4 T cells. Mice in which all but 5 percent of MHC class II molecules were bound by a single peptide had wild-type numbers of CD4 T cells. However, when the diversity within this 5 percent was lost, CD4 T cell development was impaired. Blocking the major peptide–MHC complex in thymus organ culture had no effect on T cell development, indicating that positive selection occurred on the diverse peptides present at low levels. This requirement for peptide diversity indicates that the interaction between self peptides and T cell receptors during positive selection is highly specific.
Keywords
Major histocompatibility complexPeptideBiologyT-cell receptorReceptorPopulationNegative selectionCell biologyMHC class IT cellSelection (genetic algorithm)Molecular biologyAntigenImmunologyGeneticsBiochemistryImmune systemGeneMedicine
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Publication Info
- Year
- 1999
- Type
- article
- Volume
- 283
- Issue
- 5398
- Pages
- 67-70
- Citations
- 146
- Access
- Closed
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Cite This
Gregory M. Barton,
Alexander Y. Rudensky
(1999).
Requirement for Diverse, Low-Abundance Peptides in Positive Selection of T Cells.
Science
, 283
(5398)
, 67-70.
https://doi.org/10.1126/science.283.5398.67
Identifiers
- DOI
- 10.1126/science.283.5398.67