Abstract

Caenorhabditis elegans let-7 , a founding member of the microRNA family, is predicted to bind to six sites in the 3′UTR of the mRNA of its target gene, lin-41 , to down-regulate LIN-41. Here, we demonstrate that wild-type let-7 microRNA binds in vitro to RNA from the lin-41 3′UTR. This interaction is dependent on two conserved let-7 complementary sites (LCSs). A 27-nucleotide sequence between the LCSs is also necessary for down-regulation in vivo. LCS mutations compensatory to the lesion in let-7(n2853) can partially restore lin-41 3′UTR function in a let-7(n2853) background, providing the first experimental evidence for an animal miRNA binding directly to its validated target in vivo.

Keywords

BiologyUntranslated regionCaenorhabditis elegansThree prime untranslated regionmicroRNAGeneticsMessenger RNAGeneFive prime untranslated region

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Publication Info

Year
2004
Type
article
Volume
18
Issue
2
Pages
132-137
Citations
444
Access
Closed

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Cite This

Monica C. Vella, Eun Young Choi, Shin-Yi Lin et al. (2004). The <i>C. elegans</i> microRNA <i>let-7</i> binds to imperfect <i>let-7</i> complementary sites from the <i>lin-41</i> 3′UTR. Genes & Development , 18 (2) , 132-137. https://doi.org/10.1101/gad.1165404

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DOI
10.1101/gad.1165404