Abstract
The heterchronic gene lin-14 controls the temporal sequence of developmental events in the Caenorhabditis elegans postembryonic cell lineage. It encodes a nuclear protein that normally is present in most somatic cells of late embryos and L1 larvae but is absent at later stages. Two lin-14 gain-of-function mutations delete 3'-untranslated sequences causing an inappropriately high level of the lin-14 nuclear protein late in development. These mutations identify a negative regulatory element that controls the formation of the lin-14 protein temporal gradient. The 21-kb lin-14 gene is differentially spliced to generate three lin-14 transcripts that encode protein products with variable amino-terminal regions and a constant carboxy-terminal region. The sequence of the gene revealed no protein sequence similarity to any proteins in various data bases.
Keywords
BiologyCaenorhabditis elegansGeneticsUntranslated regionGeneCaenorhabditisMessenger RNA
Affiliated Institutions
Related Publications
The C. elegans heterochronic gene lin-4 encodes small RNAs with antisense complementarity to lin-14
lin-4 is essential for the normal temporal control of diverse postembryonic developmental events in C. elegans. lin-4 acts by negatively regulating the level of LIN-14 protein, ...
A bulged lin-4/lin-14 RNA duplex is sufficient for Caenorhabditis elegans lin-14 temporal gradient formation.
The Caenorhabditis elegans heterochronic gene lin-14 generates a temporal gradient of the LIN-14 proteins to control stage-specific patterns of cell lineage during development. ...
The <i>C. elegans</i> microRNA <i>let-7</i> binds to imperfect <i>let-7</i> complementary sites from the <i>lin-41</i> 3′UTR
Caenorhabditis elegans let-7 , a founding member of the microRNA family, is predicted to bind to six sites in the 3′UTR of the mRNA of its target gene, lin-41 , to down-regulate...
Heterochronic Mutants of the Nematode <i>Caenorhabditis elegans</i>
Mutations in the Caenorhabditis elegans genes lin-14 , lin-28 , and lin-29 cause heterochronic developmental defects: the timing of specific developmental events in several tiss...
Control of Developmental Timing by MicroRNAs and Their Targets
▪ Abstract In Caenorhabditis elegans the timing of many developmental events is regulated by heterochronic genes. Such genes orchestrate the timing of cell divisions and fates a...
Publication Info
- Year
- 1991
- Type
- article
- Volume
- 5
- Issue
- 10
- Pages
- 1813-1824
- Citations
- 209
- Access
- Closed
External Links
Social Impact
Altmetric
PlumX Metrics
Social media, news, blog, policy document mentions
Citation Metrics
209
OpenAlex
Cite This
Bruce Wightman,
Thomas R. Bürglin,
Jared Gatto
et al.
(1991).
Negative regulatory sequences in the lin-14 3'-untranslated region are necessary to generate a temporal switch during Caenorhabditis elegans development..
Genes & Development
, 5
(10)
, 1813-1824.
https://doi.org/10.1101/gad.5.10.1813
Identifiers
- DOI
- 10.1101/gad.5.10.1813