Whole-Genome Random Sequencing and Assembly of Haemophilus influenzae Rd

Robert Fleischmann; Mark D. Adams; Owen White; Rebecca A. Clayton; Ewen F. Kirkness; Anthony R. Kerlavage; Carol J. Bult; Jean-François Tomb; Brian Dougherty; Joseph M. Merrick; Keith McKenney; Granger Sutton; Will FitzHugh; Chris Fields; Jeannine D. Gocayne; John Scott; Robert B. Shirley; Li-lng Liu; Anna Glodek; Jenny M. Kelley; Janice F. Weidman; Cheryl A. Phillips; Tracy Spriggs; Eva Hedblom; Matthew Cotton; Teresa R. Utterback; Michael C. Hanna; David T. Nguyen; Deborah M. Saudek; Rhonda Brandon; Leah D. Fine; Janice L. Fritchman; Joyce L. Fuhrmann; N. S. M. Geoghagen; Cheryl L. Gnehm; Lisa McDonald; Keith V. Small; Claire M. Fraser; Hamilton O. Smith; J. Craig Venter

doi:10.1126/science.7542800

Abstract

An approach for genome analysis based on sequencing and assembly of unselected pieces of DNA from the whole chromosome has been applied to obtain the complete nucleotide sequence (1,830,137 base pairs) of the genome from the bacterium Haemophilus influenzae Rd. This approach eliminates the need for initial mapping efforts and is therefore applicable to the vast array of microbial species for which genome maps are unavailable. The H. influenzae Rd genome sequence (Genome Sequence DataBase accession number L42023) represents the only complete genome sequence from a free-living organism.

Keywords

GenomeWhole genome sequencingBiologyGeneticsHaemophilus influenzaeDNA sequencingSequence assemblyComputational biologySequence (biology)Reference genomeChromosomeGeneBacteria

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Publication Info

Year: 1995
Type: article
Volume: 269
Issue: 5223
Pages: 496-512
Citations: 5563
Access: Closed

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APA Style

                            
                                
                                    Robert Fleischmann, 
                                
                                    Mark D. Adams, 
                                
                                    Owen White
                                
                                et al.
                            
                            (1995). 
                            Whole-Genome Random Sequencing and Assembly of <i>Haemophilus influenzae</i> Rd. 
                            Science
                            , 269
                            (5223)
                            , 496-512.
                            https://doi.org/10.1126/science.7542800
                        

Identifiers

DOI: 10.1126/science.7542800